Peptide Dosage Calculator

The free, fast, and accurate online tool for all your peptide calculations.

Start Your Peptide Calculation

Which syringe are you using?
0.3 mL syringe
Your Peptide Vial

What's the total amount of peptide in it?

How much bacteriostatic water are you adding?

This determines the solution's concentration.

Your Desired Dose

How much peptide do you want per injection?

Your Injection Dose

To get your 50 mcg dose with your selected 0.3 mL syringe:

Pull to 1 unit.

0.3 mL syringe scale

Zoomed-in for Accuracy:

Full size of 30units syringe scale
Swipe to explore

Peptide Reconstitution Guide

Before you can use your calculator, your peptide—which typically comes as a stable, freeze-dried powder—must be dissolved in a liquid. This process is called "reconstitution." Doing it correctly is the most critical step to ensure your peptide retains its full potency and delivers accurate results in your research. This guide will walk you through the process safely, step by step.

Prepare Your Clean Workspace

Think of this as setting up a mini-lab. Your goal is absolute sterility to ensure the purity of your research. Start by washing your hands thoroughly. Wear sterile gloves and safety goggles. Ensure your workspace is impeccably clean and gather all your essential tools:
  • Your peptide vial (freeze-dried powder)
  • Bacteriostatic water (your sterile solvent)
  • A sterile syringe for mixing (typically 3 mL)
  • A sterile insulin syringe for final dosing (e.g., 1 mL)
  • Alcohol swabs for disinfection

Prepare Your Materials

For best results, both your peptide vial and the bacteriostatic water should be at room temperature before you begin. This helps the peptide dissolve more easily and completely.

Next, carefully remove the protective cap from both vials. Use a fresh alcohol swab to thoroughly disinfect the rubber stopper on top of each vial. This simple step is vital for maintaining a sterile environment.

The Art of Adding Water

This is where precision matters. Using your sterile 3 mL mixing syringe, carefully draw your planned amount of bacteriostatic water (e.g., 1 mL or 2 mL).

The key is to be gentle. Insert the needle into the peptide vial's rubber stopper and angle it so the water slowly runs down the inside wall of the glass. Do not spray the water directly onto the peptide powder. This gentle method prevents foaming and protects the delicate peptide molecules. Once all the water is in, gently roll the vial between your fingers until all the powder is dissolved. Never shake the vial vigorously.

A Note on Concentration & Our Calculator's Pro-Tip

You now have a solution with a specific concentration (e.g., 5 mg of peptide in 1 mL of water). This concentration is what our Online Peptide Calculator uses to give you a precise unit mark on your syringe.

Here's a pro-tip for maximum accuracy: sometimes, the math results in a hard-to-measure decimal on the syringe. Our calculator is smart enough to detect this. It may offer you a "Pro-Tip" suggesting a slightly different, easy-to-measure water volume (like 1.2 mL instead of 1.0 mL). Don't worry, this doesn't change your final peptide dose—it just makes the volume you draw easier to hit perfectly on the syringe. This is a unique feature designed to make your dosing as simple and accurate as possible.

Storing Your Peptides Safely

Proper storage is crucial for maintaining your peptide's integrity, both before and after reconstitution. Here are the general scientific guidelines:
  • Unreconstituted Powder: For short-term storage, keep the vial in the refrigerator (approx. +4 °C). For long-term stability (months to years), store it in a freezer (at or below -20 °C).
  • Reconstituted Solution: Once dissolved, the solution must always be kept refrigerated (approx. +4 °C). Most peptide solutions remain stable for several weeks. To maximize shelf-life, avoid repeated freeze-thaw cycles.
  • For Exact Timelines: The precise shelf-life can vary by peptide. For the most accurate storage information, always refer to the documentation provided by your supplier.

Answers to Your Questions

Our peptide dosing calculator uses standard, medically-accepted formulas for mathematical accuracy. The final precision of your dose also relies on your own accuracy in measuring water and drawing the solution into your syringe. Our tool is designed to guide you through this process for the most reliable results.
This occurs when a precise calculation falls between the physical marks on a syringe. For these results, a icon will appear, offering guidance on how to estimate the position. Occasionally, our peptide calculator may offer a "Pro-Tip" suggesting a slight, safe adjustment to your water volume to help you land on a clear, exact line.
This calculator is designed for standard U-100 insulin syringes (0.3 mL, 0.5 mL, and 1.0 mL). The main difference is the spacing of the unit marks; smaller syringes often make it visually easier to measure precise doses. Our tool works perfectly with all three.
"U-100" is the global standard for insulin concentration, meaning there are 100 units per milliliter (mL). This universal standard is a critical safety measure, allowing for consistent and accurate dosing worldwide. It is officially documented in authoritative sources like the U.S. Pharmacopeia (USP)and enforced by regulatory bodies such as the FDA. All calculations on our site are based on this U-100 standard.
While our calculator works with any reasonable volume, 1 mL to 2 mL is most common. This creates a solution that's easy to measure and comfortable to inject. Remember, changing the water volume only changes the concentration, not the total amount of peptide, and our peptide dosing calculator accounts for this automatically.
The first step is "reconstitution," which means dissolving the freeze-dried peptide powder in a liquid like bacteriostatic water. This must be done gently to create the solution you'll be calculating your dose from. Our peptide calculator is the perfect tool for that next step.
This page features our primary calculator for standard mg/mcg dosing. For more advanced needs, we offer two specialized tools:
Your privacy is our top priority. All calculations are performed directly in your browser. We do not see, record, or store any of the data you enter. Your session is completely private and anonymous. For more details, you can review our full Privacy Policy.